The following elemental analysis and imaging equipment is available within QBIC. To send samples or sign up for training contact Keith MacRenaris.
If you need FOB access to elemental analysis equipment in Silverman Hall B540 you will need to fill out a Silverman Hall Access Form.
If you need FOB access to imaging equipment in Silverman Hall 1567 you will need to fill out a CAMI Access Form.
Elemental Analysis/Imaging (Silverman Hall B540)
Thermo Element2 High Resolution Magnetic Sector Field ICP-MS
The Thermo Element2 HR-ICP-MS (Installed October 2016) is a magentic sector field inductiveley coupled plasma mass spectrometer for the detection of most elements as low as ppq-ppt concentration range (0.010 ppq – 10 ppb). Researchers can be trained in instrument operation, sample preparation, data analysis, and basic troubleshooting. Alternatively, samples can be prepared, run, and analyzed by expert QBIC staff.
Uses < 0.5 mL sample/runFeatures:
1. Equipped with a ESI SC-2DX PrepFAST autosampler/autodilution system capable of using as little as 0.5 mL of sample per run as well as performing dilutions up to 200-fold and online internal standardization 2. Pulse counting and analog SEM detectors for covering concentration range up to 9 orders of magnitude 3. Magnetic sector field and slit selection allows for essentially interference free elemental analysis 4. Ultra-trace isotope ratio analysis is also possible
Thermo iCAP Q Inductively Coupled Plasma Mass Spectrometry (ICP-MS)
The Thermo iCAP Q ICP-MS (Installed September 2014) is a quadrupole-based inductiveley coupled plasma mass spectrometer for the detection of most elements in the ppt-ppb concentration range (0.001 – 1000 ppb). Researchers can be trained in instrument operation, sample preparation, data analysis, and basic troubleshooting for unaccompanied instrument use or samples can be prepared, run, and analyzed by the facility.
Uses ~ 2.5 mL sample/runFeatures:1. Equipped with a CETAC ASX260 120-position (15-mL conical tubes) autosampler2. Pulse counting and analog detectors for covering a concentration range of up to 9 orders of magnitude3. Collision Cell Technology (CCT) to reduce polyatomic interferences for V, Cr, Fe, As, and Se
4. Computer controlled through fully integrated QTEGRA software
Thermo iCAP 7600 Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES)
The Thermo iCAP 7600 ICP-OES (Installed October 2014) is a dual view (Radial and Axial) optical emission spectrometer used for the detection of most elements in the ppb-ppm concentration range (0.001 – 100 ppm). Can handle high matrix and total dissolved solids (TDS) up to 20%. Researchers can be trained in instrument operation, sample preparation, data analysis, and basic troubleshooting for unaccompanied instrument use or samples can be prepared, run, and analyzed by the facility.
Uses ~ 5 mL sample/runFeatures:
Perkin Elmer AAnalyst 800 Atomic Absorption Spectroscopy (AAS)
The PerkinElmer AAnalyst 800 AAS (Installed February 2011) is available for the elemental analysis of low volume samples. The AAnalyst 800 features both a graphite furnace and flame atomization which can be automatically exchanged within the instrument at the push of a button. Researchers can be trained in instrument operation, sample preparation, data analysis, and basic troubleshooting for unaccompanied instrument use or samples can be prepared, run, and analyzed by the facility.Features:
4. THGA tube for uniform temperature distribution & low interference during pyrolisis
5. Longitudinal Zeeman background correction and automatic lamp alignment.
NewWave UP213 Laser Ablation System
The ICP-MS can be coupled to the NewWave UP-213 Laser Ablation (LA) (Installed October 2008) to produce 2D elemental maps. The LA system uses a 213 nm deep UV laser to ablate a wide range of materials, from tissue specimens to geological samples. The aerosol produced is efficiently transported from the LA platform into the ICP-MS for elemental detection. Visit the Photo Gallery page for more examples of LA-ICP-MS. The LA-ICP-MS is staff run only and is not available for unaccompanied use.Features:
Milestone EthosEZ Microwave Digestion System
The Milestone EthosEZ Microwave Digestion System (Installed December 2010) is designed for closed vessel acid digestion for hard to digest samples such as polymers, ceramics, tissues, organs, soils, and sediments. Digestions are carried out in a fraction of the time when compared to heat blocks or other techniques. Quartz and Teflon inserts are available for microliter sample sizes and are HF compatible.Features:
Microscopy (Silverman Hall 1567)
Zeiss 510 LSM Inverted Confocal Microscope
The Inverted Zeiss Axio Observer.Z1 Confocal Microscope (Installed September 2008) is ideal for a wide range of applications, including biochemistry, medical and material sciences. The fluorescence microscope produces high resolution z-slice images with 3-D reconstruction over a wide range of wavelengths from UV to Near IR. The Axio Observer.Z1 is completely digital providing added sensitivity and performance for exceptional quality images.Features:
Zeiss 510 LSM Upright Confocal Microscope
The Zeiss LSM 510 Upright Confocal Microscope (Installed June 2003) is equipped with multiple laser lines for the excitation of virtually every fluorophore. By design, the upright confocal provides an ideal platform with dipping objectives for imaging of solvent immersed samples. The microscope is also equipped with a tunable Meta detector with multi-wavelength detection capabilities as well as a 2-photon laser.Features:1. Excitation: Ar (458, 488, 514 nm), HeNe (543 nm), HeNe (633 nm), 2-photon (690-1024 nm) 2. Objectives: 2.5x (air, NA – 0.12, WD – 0.17 mm), 10x (air, NA – 0.3, WD – 0.17 mm), 20x (air, NA – 0.75, WD – 0.17 mm), 63x (oil, NA – 1.4, WD – 0.17 mm), and 63 x (dipping, NA – 1.4, WD – 0.17 mm) 3. Equipped with a Meta spectral detector which provides emission wavelength spectra as well as serving as a variable bandpass filter 4. Equipped with 3 additional filter-based PMT detectors allow for the simultaneous separation of multiple fluorescent signals 5. Computer controlled using Zen2007 software, equipped with large HP LP3065 30-inch display 6. Specifically designed for multi-spectral imaging and spectral un-mixing, However with no incubation or CO2 control systme is not well suited for imaging other than at room temperature
Spectra Physics Mai Tai DeepSee 2-Photon Laser
Both the Upright Axio Observer.Z1 and the LSM 510 Meta are interfaced with a digitally controlled 2-photon laser (Spectra Physics Mai Tai). This tunable Near IR pulsed laser is capable of providing excitation over the full UV and visible range for simultaneous excitation of multiple fluorophores (690 nm – 1040 nm). 2-photon imaging is ideal for live, deep-tissue microscopy by providing a low energy excitation with reduced phototoxicity.
Olympus IX83 Inverted Fluorescent Microscope
The Olympus IX83 Inverted Fluorescent Microscope (Installed June 2014) is an inverted fluorescence microscope equipped with motorized z height adjustment capabilities, a high frame rate (30 fps) ANDOR Xyla camera and long working distance objectives for imaging cells, tissues and materials samples on glass or plastic (i.e. multi-well plates) substrates.Features:
Olympus IX53 Inverted Color Microscope (w/ Micro-injection/Micro-manipulation)
The Olympus IX53 Inverted Color Microscope (Installed June 2014) is an inverted microscopeFeatures:1. Objectives: 2.5x (air, NA – , WD – ), 10x (air, NA – 0.3, WD – 9.37 mm), 20x (air, NA – 0.45, WD – 6.4-7.6 mm), 40x (air, NA – 0.6, WD – 2.7-4.0) 2. Equipped with an Eppendorf Transferman NK2 Micro-Manipulation System 3. Equipped with a Warner Instruments PLI-100A Picoinjector 4. Hoffman printed objectives for better viewing in plastic dishes and multi-well plates 5. Long Working Distance universal condenser (NA – 0.5, WD – 45 mm) 6. LC20 2.0 MP color camera with Cellsens Entry software
NUANCE-EPIC (Silverman Hall B540)
Hitachi HD-2300 Scanning Transmission Electron Microscope (STEM)
The Hitachi HD-2300 STEM is equipped with dual EDX (x-ray Energy Dispersive Spectroscopy) detectors for enhanced sensitivity. The microscope also features the Gatan Enfina 2000, electron energy-loss spectrometer (EELS). Samples can be imaged by secondary electron, Z-contrast (annular darkfield), or transmitted electron (brightfield) detectors. For more information about STEM imaging and cryo sample preparation and imaging contact Reiner Bleher or visit the EPIC website.